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Lactating Nili-Ravi Buffaloes were injected rbST (500 mg/16 day) to observe effect on milk production, composition, physiological profile, minerals of serum, milk and mammary health biomarkers. Respiration rate was significantly high after day 1 of first injection and low during days 24, 28 and 32 of second injection irrespective of their groups. Overall mean ESR, neutrophils percent decreased and lymphocytes percent increased significantly in bST treated as compared to control buffaloes. The overall increase in milk production was 9.31% from bST treated buffaloes. Overall mean milk lactose increased significantly and milk fat was 1.03% higher (P 0.05) in bST treated buffaloes. Milk calcium, magnesium, sodium, chloride and phosphorus increased significantly. Serum calcium and sodium did increase (P 0.05) while magnesium, potassium, chloride, and phosphorus did decrease (P 0.05) in bST injected buffaloes. A significant increase in milk plasminogen and a decrease in milk plasmin was observed in bST treated buffaloes. Serum ALT, paraoxonase, MDA were significantly high and alkaline phosphatase and the AOS decreased significantly in bST injected buffaloes.

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Dr. Tanweer Khaliq is working as Assistant Professor in the Department of Physiology and Pharmacology, University of Agriculture, Faisalabad, Pakistan. He has 27 years experience of teaching and research and published 18 research papers in national and international journals.

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