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Focal segmental glomerulosclerosis "(FSGS)" is responsible in the majority of renal patients for progression to end-stage renal disease (D´Agati VD et al. 2011). Considering that parietal and visceral epithelial cells play important roles in the pathogenesis of rapidly progressive glomerulonephritis "(RPGN)" and FSGS, the major aim of this work was to provide a highly efficient and reliable approach for isolation and generation of a primary culture of parietal epithelial cells and podocytes from two different transgenic mice.

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