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Zu dem Thema gibt es viele Publikationen, die von Experten für Experten geschrieben wurden. Dieses Buch wendet sich insbesondere an Studenten höherer Semester und Forscher, denen das Hintergrundwissen der Physik fehlt, um neuartige Verfahren der Fluoreszenzmikroskopie zu verstehen.
Die zweite Auflage wartet mit neuen Kapiteln und einer erweiterten Einführung auf. Der Schwerpunkt liegt auf der hochauflösenden und Einzelmolekül-Mikroskopie. Jedes Kapitel wurde von einem anerkannten Experten des Fachgebiets geschrieben und sorgfältig überarbeitet, um so die Entwicklungen der letzten Jahre wiederzugeben.

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While there are many publications on the topic written by experts for experts, this text is specifically designed to allow advanced students and researchers with no background in physics to comprehend novel fluorescence microscopy techniques.
This second edition features new chapters and a subsequent focus on super-resolution and single-molecule microscopy as well as an expanded introduction. Each chapter is written by a renowned expert in the field, and has been thoroughly revised to reflect the developments in recent years.

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Introduction to Optics
(Rainer Heintzmann and Ulrich Kubitscheck)
Principles of Light Microscopy
(Ulrich Kubitscheck)
Fluorescence Microscopy
(Jurek W. Dobrucki and Ulrich Kubitscheck)
Fluorescence Labeling
(Gerd Ulrich Nienhaus and Karin Nienhaus)
Confocal Microscopy
(Nikolaus Naredi-Rainer, Jens Prescher, Achim Hartschuh and Don C. Lamb)
Two-Photon Excitation Microscopy for Three-Dimensional Imaging of Living Intact Tissues
(David W. Piston)
Light Sheet Microscopy
(Gopi Shah, Michael Weber and Jan Huisken)
Localization-Based Super-Resolution Microscopy
(Markus Sauer and Mike Heilemann)
Super-Resolution Microscopy: Interference and Pattern Techniques
(Udo Birk, Gerrit Best, Roman Amberger and Christoph)
STED Microscopy
(Travis J. Gould, Lena K. Schroeder, Patrina A. Pellett and Joerg Bewersdorf)
Fluorescence Photobleaching Techniques
(Reiner Peters)
Single-Molecule Microscopy in the Life Sciences
(Markus Axmann, Josef Madl and Gerhard J. Schütz)
Förster Resonance Energy Transfer and Fluorescence Lifetime Imaging
(Fred S. Wouters)
Appendix A: What Exactly is a Digital Image?
Ulrich Kubitscheck
Appendix B: Practical Guide to Optical Alignment
(Rainer Heintzmann)
Index

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Ulrich Kubitscheck is the department head of Biophysical Chemistry at the Institute of Physical and Theoretical Chemistry at the University of Bonn, Germany. Having obtained his academic degrees from the University of Bremen, Germany, he spent his career working at The Weizmann Institute of Science and the Institute of Medical Physics and Biophysics of the University of Münster, Germany, before taking up his present appointment at the University of Bonn. Professor Kubitscheck develops single molecule and light sheet imaging techniques, has authored over 80 scientific publications and has extensive experience in teaching courses on physical chemistry, biophysics and quantitative microscopy.

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