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This detailed volume presents a comprehensive technical overview of DNA nanotechnology with an emphasis on 3D DNA nanostructure design and applications. Coverage spans from basic design principles for DNA and RNA nanostructures to their cutting-edge applications in a variety of fields, with the book divided into basic DNA and RNA nanostructure design strategies as well as applications utilizing DNA nanostructures, including but not limited to nanomedicine, bioimaging, biosensing, nanoplasmonics, nanoelectronics, nanofabrication, crystallography, biophysics, and analytical chemistry. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, 3D DNA Nanostructure: Methods and Protocols provides the most up-to-date tutorial style overviews and technical style protocols to benefit researchers in a wide variety of areas.

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Part I: DNA/RNA Structure Design

1. Designed 3D DNA Crystals

Nadrian C. Seeman, Ruojie Sha, Jens Birktoft, Jianping Zheng, Wenyan Liu, Tong Wang, and Chengde Mao

2. Three-Dimensional DNA Nanostructures Assembled from DNA Star Motifs

Cheng Tian and Chuan Zhang

3. Design of Wireframe DNA Nanostructures-DNA Gridiron

Dongran Han

4. Complex DNA Brick Assembly

Luvena Ong, Yonggang Ke, and Peng Yin

5. Computer-Aided Design of RNA Origami Structures

Steffen L. Sparvath, Cody W. Geary, and Ebbe S. Andersen

6. Assembling RNA Nanoparticles

Shou-Jun Xiao

Part II: Applications of DNA Structures

7. DNA Functionalization of Nanoparticles

Fang Lu and Oleg Gang

8. Purification Techniques for Three-Dimensional DNA Nanostructures

Travis A. Meyer

9. DNA Nanostructure as Smart Carriers for Drug Delivery

Xiangyuan Ouyang, Jie Chao, Shao Su, and Chunhai Fan

10. DNA G-Quadruplex-Based Assay of Enzyme Activity
Zhuoliang Liu, Kaiyu He, Wang Li, Xin Liu, Xiahong Xu, Zhou Nie, and Shouzhuo Yao

11. Assembly of Multi-Enzyme Cascade on a DNA Origami Nanostructure

Jinglin Fu and Tianran Li

12. Lipid Membrane Encapsulation of a 3D DNA NanoOctahedron

Steven D. Perrault and William M. Shih

13. DNA-PAINT Super-Resolution Imaging for Nucleic Acid Nanostructures

Mingjie Dai

14. Designing DNA Nanotube Liquid Crystals as a Weak-Alignment Medium for NMR Structure Determination of Membrane Proteins

John Min, William M. Shih, and Ga‰tan Bellot

15. Direct Nanofabrication Using DNA Nanostructure

Feng Zhou and Haitao Liu

16. Confined Growth of Metal Nanoparticles Within 3D DNA Origami Molds

Wei Sun and Jie Shen

17. DNA-Directed Self-Assembly of Highly Ordered and Dense Single Walled Carbon Nanotube Arrays

Hareem Maune and Si-ping Han

18. A Proximity-Based Programmable DNA Nanoscale Assembly Line

Xiaoyan Zhang, Xiaoqiang Ding, Jianzhou Zou, and Hongzhou Gu

19. DNA Walkers as Transport Vehicles of Nanoparticles Along a Carbon Nanotube Track

Jing Pan, Tae-Gon Cha, Haorong Chen, Feiran Li, and Jong Hyun Choi

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