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This volume includes comprehensive descriptions of miRNA biogenesis and their role in the development and progression of various human diseases. The first few chapters of MicroRNA Profiling: Methods and Protocols discuss the effects of over-expressing and repressing of a target miRNA and their effects on cell viability and proliferation. The next few chapters explore the protocols for total RNA isolation from cells and cell-derived product including formalin fixed paraffin embedded tissue and plant tissue. The last few chapters discuss isolation and characterization of exosomes from medium conditioned by cell lines, serum, and plasma specimens. This book also includes discussions of several software tools, such as miRandola, PicTar, DIANA, and miRWalk. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, MicroRNA Profiling: Methods and Protocols is a valuable resource for anyone interested in the field of Micro RNAs.

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1. MiRNA Biogenesis and Regulation of Diseases: An Overview
Anchal Vishnoi and Sweta Rani

2. Assessment of Basic Biological Functions Exerted by miRNAs
John Nolan, Raymond L. Stallings, and Olga Piskareva

3. Extraction of miRNAs from Formalin Fixed Paraffin Embedded (FFPE) Tissues
Karen Howe

4. MiRNA Isolation from Plants Rich in Polysaccharides and Polyphenols
K.K. Sabu, F. Nadiya, and N. Anjali

5. MicroRNA Profiling of Exosomes
Melissa Daly and Lorraine O´Driscoll

6. MiRNA Profiling in Human Induced Pluripotent Stem Cells
Erica Hennessy
7. MiRNA Expression in Cystic Fibrosis Bronchial Epithelial Cells
Irene K. Oglesby and Paul J. McKiernan

8. TaqMan Low Density Array: MicroRNA Profiling for Biomarker and Oncosuppressor Discovery
Keith O´Brien

9. Detection of MicroRNAs in Brain Slices Using In Situ Hybridization
Sean Quinlan, Christine Henke, Gary P. Brennan, David C. Henshall, and Eva M. Jimenez-Mateos

10. Exosomal MicroRNA Discovery in Age-Related Macular Degeneration
Hanan Elshelmani and Sweta Rani

11. Profiling the MicroRNA Payload of Exosomes Derived from Ex Vivo Primary Colorectal Fibroblasts
R. Bhome, R. Goh, K. Pickard, M. Mellone, A.E. Sayan, and A. Mirnezami

12. Circulating MicroRNAs in Cancer
Killian P. O´Brien, Eimear Ramphul, Linda Howard, William M. Gallagher, Carmel Malone, Michael J. Kerin, and Róisín M. Dwyer

13. Profiling Circulating MiRNAs from the Plasma of Individuals with Metabolic Syndrome
Sadhbh O´Neill and Lorraine O´Driscoll

14. Manipulating MiRNA Expression to Uncover Hidden Functions
Sin éad T. Aherne and Nga T. Lao

15. Analysis of the Distribution Profiles of Circulating MicroRNAs by Asymmetrical Flow Field Flow
Fractionation
Kenneth Flack, Luis Jimenez, Wenwan Zhong

16. MicroRNA Expression Profiling Using Agilent One-Color Microarray
Carmela Dell´Aversana, Cristina Giorgio, and Lucia Altucci

17. A Multiplex Ligation Assay for MiRNA Copy Number Profiling
Duncan Kilburn, Yunke Song, Tza-Huei Wang, and Kelvin J. Liu

18. Practical Bioinformatics Analysis of MiRNA Data Using Online Tools
James A.L. Brown and Emer Bourke

19. Visualization and Analysis of MiRNA-Targets Interactions Networks
Luis E. L eón and Sebastián D. Calligaris

20. Guidelines on Designing MicroRNA Sponges: From Construction to Stable Cell Line
Manoela Marques Ortega and Hakim Bouamar

21. Customization of Artificial MicroRNA Design
Tien Van Vu and Vinh Nang Do

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