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This volume provides readers with detailed protocols covering the main cancer cytogenetics techniques needed for clinical utilization and research purposes. The chapters in this book cover topics such as chromosome preparation for myeloid malignancies; chromosome bandings; fluorescence in situ hybridization probe preparation; array-based comparative genomic hybridization; and cytogenetic nomenclature and reporting. The updated reviews on chromosomal abnormalities in hematological malignancies are excellent guides for cytogenetics data interpretations and specific malignant diseases correlation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Cancer Cytogenetics: Methods and Protocols is a valuable resource for the novice in cytogenetics because it provides helpful guiding protocols, but it´s also great for those who are already engaged in the field and are looking for some technical hints.

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Preface...
Table of Contents...
Contributing Authors...

1. Cancer Cytogenetics: An Introduction

Thomas S.K. Wan

2. Chromosome Preparation for Myeloid Malignancies

Eleanor K.C. Hui, Thomas S.K. Wan, and Margaret H.L. Ng

3. Chromosome Preparation for Acute Lymphoblastic Leukemia
Mary Shago
4. Chromosome Preparation for Chronic Lymphoid Malignancies
Dorota Koczkodaj and Agata A. Filip
5. Cytogenetic Harvesting of Cancer Cells and Cell Lines

Roderick A.F. MacLeod, Maren E. Kaufmann, and Hans G. Drexler

6. Chromosome Bandings

Huifang Huang and Jiadi Chen

7. Chromosome Recognition

Thomas S.K. Wan, Eleanor K.C. Hui, and Margaret H.L. Ng

8. Application of Fluorescence In Situ Hybridization Technology in Malignancies

Montakarn Tansatit

9. Fluorescence In Situ Hybridization Probe Preparation

Doron Tolomeo, Roscoe R. Stanyon, and Mariano Rocchi
10. Fluorescence In Situ Hybridization Probe Validation for Clinical Use

Jun Gu, Janice L. Smith, and Patricia K. Dowling

11. Fluorescence In Situ Hybridization on Tissue Sections

Alvin S.T. Lim and Tse Hui Lim
12. Cytoplasmic Immunoglobulin Light Chain Revelation and Interphase Fluorescence In Situ Hybridization in Myeloma

Sarah Moore, Jeffrey M. Suttle, and Mario Nicola
13. Quantitate Fluorescence In Situ Hybridization (QFISH)

Ivan Iourov

14. High Resolution of Fiber-Fluorescence In Situ Hybridization (Fiber-FISH)

Christine J. Ye and Henry H. Heng

15. Array-Based Comparative Genomic Hybridization (aCGH)
Chengsheng Zhang, Eliza Cerveira, Mallory Romanovitch, and Qihui Zhu
16. Multicolor Karyotyping and Fluorescence In Situ Hybridization-Banding (MCB/mBAND)

Thomas Liehr, Moneeb A.K. Othman, and Katharina Rittscher

17. Cytogenetics for Biological Dosimetry

Michelle Ricoul, Tamizh Gnana-Sekaran, Laure Piqueret-Stephan, and Laure Sabatier

18. Recurrent Cytogenetic Abnormalities in Myelodysplastic Syndromes

Meaghan Wall

19. Recurrent Cytogenetic Abnormalities in Acute Myeloid Leukemia

John J. Yang, Tae Sung Park, and Thomas S.K. Wan

20. Recurrent Cytogenetic Abnormalities in="" myeloproliferative="" neoplasms="" and="" chronic="" myeloid="" leukemia
John Swansbury

21. Recurrent Cytogenetic Abnormalities in Acute Lymphoid Leukemia

Mary Shago

22. Recurrent Cytogenetic Abnormalities in Non-Hodgkin´s Lymphoma and Chronic Lymphocytic Leukemia

Edmond S.K. Ma

23. Recurrent Cytogenetic Abnormalities in Multiple Myeloma

Nelson C.N. Chan and Natalie P.H. Chan

24. Cytogenetic Nomenclature and Reporting

Marian Stevens-Kroef, Annet Simons, Katrina Rack, and Rosalind J. Hastings

25. Cytogenetic Resources and Information

E. De Braekeleer, J. L. Huret, H. Mossafa, and P. Dessen

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