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This volume provides detailed discussion of a variety of important techniques that researchers use to study fungal molecular biology and pathogenesis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Candida Species: Methods and Protocols aims to ensure successful results in the further study of this vital field.

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PART I: Molecular Analyses Of Pathogenesis

1 How to use the Candida Genome Database

Marek S. Skrzypek, Jonathan Binkley, and Gavin Sherlock

2 Transcriptional Profiling of Candida albicans in the Host

Kearney T.W. Gunsalus, Carol A. Kumamoto

3 SRYTH: A New Yeast Two-Hybrid Method

Jaideep Mallick, Gregor Jansen, Cunle Wu and Malcolm Whiteway

4 Chromatin immunoprecipitation (ChIP) assay in Candida albicans

S reyoshi Mitra, Laxmi Shanker Rai, Gautam Chatterjee, and Kaustuv Sanyal

5 Assessing mitochondrial functions in Candida albican s

Richard Calderone and Dongmei Li

6 Imaging Candida Infections in the Host

Dhammika H. Navarathn, David D. Roberts, Jeeva Munasinghe, and Martin J. Lizak

7 Identification of Secreted Candida Proteins using Mass Spectrometry

Emilia Gómez-Molero, Henk L. Dekker, Albert D. de Boer, and Piet W.J. de Groot

8 In vitro models for Candida Biofilm Development

Bastiaan P. Krom and Hubertine M.E. Willems

9 The Fungal Biome of the Oral Cavity

Jyotsna Chandra, Mauricio Retuerto, Pranab K. Mukherjee, and Mahmoud Ghannoum

10 Experimental models of C. albicans -streptococcal Co-infection

Takanori Sobue, Patricia Diaz, Hongbin Xu, Martinna Bertolini, and Anna Dongari-Bagtzoglou

11 Staphylococcus - Candida interaction models: Antibiotic Resistance Testing and Host Interaction

Nina Scheres and Bastiaan P. Krom

PART II: Drug Discovery and Susceptibility Testing

12 Genetic Screens for Determination of Mechanism of Action

Fran‡oise Gay-Andrieu, Deepu Alex, and Richard Calderone

13 Microbroth Dilution Susceptibility Testing of Candida Species

Randal J. Kuykendall and Shawn R. Lockhart

14 Biofilms and Antifungal Susceptibility Testing

Maria Simitsopoulou, Athanasios Chatzimoschou, and Emmanuel Roilides

15 Typing Candida Species using Microsatellite Length Polymorphism and Multilocus Sequence Typing

Dea Garcia-Hermoso, Marie Desnos-Ollivier, and Stéphane Bretagne

16 Diagnostic Methods for Detection of Blood-borne Candidiasis

Cornelius J. Clancy, and M. Hong Nguyen

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