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This volume presents various laboratory protocols, reviews, specific techniques and applications related to Schizosaccharomyces pombe. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Schizosaccharomyces pombe: Methods and Protocols hopes to serve as an excellent resource for faculty researchers, undergraduate student researchers, graduate researchers, government, and the medical community.

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1. Preparation of Solutions and Reagents

Teresa L. Singleton

2. Analysis of Fission Yeast Single DNA Molecules on the Megabase Scale using DNA Combing

Atanas Kaykov

3. Chromatin Immunoprecipitation - Polymerase Chain Reaction (ChIP-PCR) Detects Methylation, Acetylation, and Ubiquitylation in S. pombe

Valerie Migeot and Damien Hermand
4. Primer Design and Inverse PCR on Yeast Display Antibody Selection Outputs
Fortunato Ferrara, Andrew R. M. Bradbury, and Sara D´Angelo

5. Molecular Cloning and Characterization of Small Viral Genome in Fission Yeast

Ge Li and Richard Y. Zhao

6. Total RNA Isolation and Quantification of Specific RNAs in Fission Yeast

Robert Roth, Hiten D. Madhani, and Jennifer F. Garcia

7. Analysis of Reverse Transcribed mRNA using PCR and Polyacrylamide Gel Electrophoresis

Pranjal Biswas, Uddalak Majumdar, and Sanjay Ghosh

8. The No-nonsens SDS-PAGE

Lars Backman and Karina Persson

9. Crystallization of Recombinant -actinin and Related Proteins

Karina Persson and Lars Backman

10. Estimation of GFP-nucleoporin Amount Based on Fluorescence Microscopy

Haruhiko Asakawa, Yasushi Hiraoka, and Tokuko Haraguchi

11. Antibody Pull-down Experiments in Fission Yeast

Qianhua Dong and Fei Li

12. Preparation of Cell Lysates of Fission Yeast for Immunoprecipitation

Akihisa Matsuyama, Atsuko Shirai, and Minoru Yoshida

13. Wide-band Electrical Impedance Spectroscopy (EIS) Measures S. pombe Cell Growth in vivo

Zhen Zhu, Olivier Frey, and Andreas Hierlemann

14. In Situ Chromatin Binding Assay Using Epifluorescent Microscopy in S . pombe

Jinpu Yang and Fei Li

15. High-frequency Lithium Acetate Transformation of Schizosaccharomyces pombe

Sudhir Kumar Rai, Angela Atwood-Moore, and Henry L Levin

16. Tetrad Dissection in Fission Yeast

Wilber Escorcia and Susan L. Forsburg

17. Random Spore Analysis in Fission Yeast

Wilber Escorcia and Susan L. Forsburg

18. Duplication and Transformation of the Schizosaccharomyces pombe Collection of Deletion Strains

Sudhir Kumar Rai, Angela Atwood-Moore, and Henry L. Levin

19. Schizosaccharomyces pombe Biotechnological Applications in Winemaking

µngel Benito, Fernando Calderón, and Santiago Benito

20. Schizosaccharomyces pombe Isolation Protocol

µngel Benito, Fernando Calderón, and Santiago Benito

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