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This vital collection provides a unique set of techniques to explore the clinical, pathological, and research aspects of the management of patients with esophageal adenocarcinoma. Beginning with an introduction to the basic features of esophageal adenocarcinoma, the book continues with clinical protocols for the management of the cancer, pathological methods for management of the patients and research, as well as protocols for molecular research, which could aid researchers in furthering our understanding of pathogenesis as well as in identifying new targets for the treatment and prevention of adenocarcinoma. Written for the highly successful Methods in Molecular Biology series, chapters include introductions on their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Esophageal Adenocarcinoma: Methods and Protocols is a valuable guide to stimulate specialists from various disciplines in their continuing research into esophageal adenocarcinoma and translating that research into improving the management of this cancer of rising importance.

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1. Introduction: Esophageal Adenocarcinoma: Updates of Current Status

Alfred K. Lam

2. Radiotherapy for Esophageal Adenocarcinoma

Dora L.W. Kwong

3. Chemotherapy for Esophageal Adenocarcinoma

Ka-On Lam and Dora L.W. Kwong

4. Surgical Protocol for Esophageal Adenocarcinoma

Simon Law

5. Target Therapy for Esophageal Adenocarcinoma

Ka-On Lam and Dora L.W. Kwong

6. Histopathological Assessment for Esophageal Adenocarcinoma

Alfred K. Lam

7. Macroscopic Assessment and Cut Up of Endoscopic Resection Specimens for Early Esophageal Glandular Malignancies

Benjamin M. Allanson and Marian P. Kumarasinghe

8. Processing of Surgical Specimen (Esophagogastrectomy) for Esophageal Adenocarcinoma

Benjamin M. Allanson and Marian P. Kumarasinghe

9. Application of Pathological Staging in Esophageal Adenocarcinoma

Alfred K. Lam

10. Application of Tissue Microarray in Esophageal Adenocarcinoma

Nassim Saremi and Alfred K. Lam

11. Predictive Marker: HER2 in Esophageal Adenocarcinoma

Duminda Subasinghe, Nathan Acott, and Marian P. Kumarasinghe

12. Whole-Slide Imaging for Esophageal Adenocarcinoma

Alfred K. Lam and Melissa Leung

13. Animal Model: Reflux Models in Esophageal Adenocarcinoma

Takahiro Masuda and Sumeet K. Mittal

14. Animal Model: Xenograft Mouse Models in Esophageal Adenocarcinoma

Md Sazzad Hassan and Urs von Holzen

15. Identification of Cancer Stem Cells in Esophageal Adenocarcinoma

Farhadul Islam, Vinod Gopalan, and Alfred K. Lam

16. Circulatory Tumor Cells in Esophageal Adenocarcinoma

Vinod Gopalan and Alfred K. Lam

17. Liquid Biopsy for Investigation of Cancer DNA in Esophageal Adenocarcinoma: Cell-Free Plasma DNA and Exosome-Associated DNA

Robert A. Smith and Alfred K. Lam

18. Somatic DNA Copy Number Alterations Detection for Esophageal Adenocarcinoma Using Digital Polymerase Chain Reaction

Katherine T.W. Lee, Vinod Gopalan, and Alfred K. Lam

19. Targeted Single Gene Mutation in Esophageal Adenocarcinoma

Katherine T.W. Lee, Robert A. Smith , Vinod Gopalan, and Alfred K. Lam

20. DNA Genome Sequencing in Esophageal Adenocarcinoma

Suja Pillai, Vinod Gopalan, and Alfred K. Lam

21. Epigenetics: DNA Methylation Analysis in Esophageal Adenocarcinoma

Farhadul Islam, Johnny C. Tang, Vinod Gopalan, and Alfred K. Lam

22. Detection and Quantification of MicroRNAs in Esophageal Adenocarcinoma

Moien Amin, Farhadul Islam, Vinod Gopalan, and Alfred K. Lam

23. RNA Interference-Mediated Gene Silencing in Esophageal Adenocarcinoma

Farhadul Islam, Vinod Gopalan, and Alfred K. Lam

24. Proteomic Protocol in Esophageal Adenocarcinoma

Peter Kelly

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