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This volume details protocols on virus-derived nanoparticles (VNPs) for a number of different applications. Chapters guide readers through the production of VNPs derived from plant, animal and bacterial viruses, prokaryotic and eukaryotic expression systems, encapsulation of heterologous materials within VNPs, and the modification of the outer surface of VNPs and how such modified VNPs can be developed into functional entities. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Virus-Derived Nanoparticles for Advanced Technologies: Methods and Protocols aims to ensure successful results in the further study of this vital field.

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PART I: PREPARING VIRUS AND VIRUS-DERIVED NANOARCHITECTURES AS CONTAINERS, CARRIER SCAFFOLDS, AND STRUCTURE-DIRECTING AGENTS: FROM PARTICLES TO FUNCTIONAL SUPER-ASSEMBLIES 1. The Production in Plants of Mosaic Turnip Crinkle Virus-like Particles Derived by Co-infiltration of Wild-Type and Modified Forms of Virus Coat Protein Roger Castells-Graells, George P. Lomonossoff and Keith Saunders
2. Isolation and Characterization of Two Distinct Types of Unmodified Spherical Plant Sobemovirus-like Particles for Diagnostic and Technical Uses Ina Balke, Gunta Resevica, and Andris Zeltins
3. RNA-directed Assembly of Tobacco Mosaic Virus (TMV)-Like Carriers with Tunable Fractions of Differently Addressable Coat Proteins Sabine Eiben
4. Fabrication of Tobacco Mosaic Virus-like Nanorods for Peptide Display Emily J. Larkin, Adam D. Brown, and James N. Culver
5. In planta Production of Fluorescent Filamentous Plant Virus-based Nanoparticles Sourabh Shukla, Christina Dickmeis, Rainer Fischer, Ulrich Commandeur, and Nicole F. Steinmetz
6. Self-assembling Plant-derived Vaccines against Papillomaviruses Emanuela Noris
7. Recombinant Expression of Tandem-HBc Virus-like Particles (VLPs) Sam L Stephen, Lucy Beales, Hadrien Peyret, Amy Roe, Nicola J. Stonehouse, and David J. Rowlands
8. Production and Application of Insect Virus-based VLPs Radhika Gopal and Anette Schneemann
9. Nanomanufacture of Free-standing, Porous, Janus-Type Films of Polymer-Plant Virus Nanoparticle Arrays Brylee David B. Tiu, Rigoberto C. Advincula, and Nicole F. Steinmetz
10. Self-assembly of Rod-like Bionanoparticles at Interfaces and in Solution Ye Tian and Zhongwei Niu
11. Bottom-up Assembly of TMV-based Nucleoprotein Architectures on Solid Supports Christina Wege and Fabian Eber
PART II: FUNCTIONAL COMPOUNDS IN VIRUS-BASED CONTAINERS: FROM PREPARATION TO APPLICATIONS OF HYBRID NANOOBJECTS 12. Internal Deposition of Cobalt Metal and Iron Oxide within CPMV eVLPs Alaa A. A. Aljabali and David J. Evans
13. Plant Virus-based Nanoparticles for the Delivery of Agronomic Compounds as a Suspension Concentrate Richard H. Guenther, Steven A. Lommel, Charles H. Opperman, and Tim L. Sit
14. Nanowires and Nanoparticle Chains inside Tubular Viral Templates Kun Zhou and Qiangbin Wang
15. In vitro-Reassembled Plant Virus-Like Particles of Hibiscus Chlorotic Ringspot Virus (HCRSV) as Nano-protein Cages for Drugs Sek-Man Wong and Yupeng Ren
16. CCMV-based Enzymatic Nanoreactors Mark V. de Ruiter, Rindia M. Putri, and Jeroen J.L.M. Cornelissen
17. Protocol for the Efficient Cell-free Synthesis of Cowpea Chlorotic Mottle Virus-like Particles Containing Heterologous RNAs Rees F. Garmann, Charles M. Knobler, and William M. Gelbart
18. Packaging DNA Origami into Viral Protein Cages Veikko Linko, Joona Mikkilä, and Mauri A. Kostiainen
19. In Vitro Assembly of Virus-Derived Designer Shells around Inorganic Nanoparticles Stella E. Vieweger, Irina B. Tsvetkova, and Bogdan G. Dragnea
20. In Vivo Packaging of Protein Cargo inside of Virus-Like Particle P22 Kimberly McCoy and Trevor Douglas
21. Encapsulation of Negatively Charged Cargo in MS2 Viral Capsids Ioana L. Aanei, Jeff E. Glasgow, Stacy L. Capehart, and Matthew B. Francis
22. Delivering Cargo: Plant-based Production of Bluetongue Virus core- and Virus-like Particles Containing Fluorescent Proteins Eva C. Thuenemann and George P. Lomonossoff
PART III: FUNCTIONS EXPOSED ON VIRUS BACKBONES AND COMBINED APPROACHES: FROM FABRICATION TO TECHNICAL USES OF HYBRID PARTICLES, FILMS, AND MATERIALS
23. Bioinspired Silica Mineralization on Viral Templates Christina Dickmeis, Klara Altintoprak, Patrick van Rijn, Christina Wege, and Ulrich Commandeur
24. Propagation and Isolation of Tobacco Mosaic Virus Which Surface Displays Metal Binding and Reducing Peptides for Generation of Gold Nanoparticles Andrew J. Love and Michael E. Taliansky
25. TMV-templated Formation of Metal and Polymer Nan

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