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This second edition volume expands on the previous edition with chapters discussing the latest developments and research initiatives in mitochondrial functions. The chapters in this book explore topics such as high-resolution fluorespirometry and OXPHOS protocols in human cells, analysis of mitochondrial oxygen consumption, mitochondrial bioenergetics, and mitochondrial dynamics in mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Mitochondrial Bioenergetics: Methods and Protocols, Second Edition is a valuable resource for students, and practitioners in the pharmaceutical sciences, environmental sciences, and mitochondrial genetics industries.

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Preface... Table of Contents... Contributing Authors...
1. Overview of Mitochondrial Bioenergetics Vitor M.C. Madeira
2. Evaluation of Respiration with Clark Type Electrode in Isolated Mitochondria and Permeabilized Animal Cells Ana M. Silva and Paulo J. Oliveira
3. High-Resolution FluoRespirometry and OXPHOS Protocols for Human Cells, Permeabilized Fibres from Small Biopsies of Muscle and Isolated Mitochondria Carolina Doerrier, Luiz F. Garcia-Souza, Gerhard Krumschnabel, Yvonne Wohlfarter, András T. Mészáros, and Erich Gnaiger
4. High-Throughput Analysis of Mitochondrial Oxygen Consumption James Hynes, Rachel L. Swiss, and Yvonne Will
5. Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal Slices Ana Ledo, Rui M. Barbosa, and JoÆo Laranjinha
6. Mitochondrial Membrane Potential (Delta ) Fluctuations Associated with the Metabolic States of Mitochondria JoÆo S. Teodoro, Anabela P. Rolo, and Carlos M. Palmeira
7. Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells David G. Nicholls
8. Comparison of Mitochondrial Incubation Media for Measurement of Respiration and Hydrogen Peroxide Production Timea Komlódi, Ondrej Sobotka, Gerhard Krumschnabel, Nicole Bezuidenhout, Elisabeth Hiller, Carolina Doerrier, and Erich Gnaiger
9. Measurement of Proton Leak in Isolated Mitochondria Charles Affourtit, Hoi-Shan Wong, and Martin D. Brand
10. Imaging Mitochondrial Calcium Fluxes with Fluorescent Probes and Single or Two Photon Confocal Microscopy Sean M. Davidson and Michael R. Duchen
11. Mitochondrial Permeability Transition Pore and Calcium Handling Randi J. Parks, Elizabeth Murphy, and Julia C. Liu
12. Redox Equivalents and Mitochondrial Bioenergetics James R. Roede, Young-Mi Go, and Dean P. Jones
13. Mitochondrial Bioenergetics by 13 C-NMR Isotopomer Analysis Rui A. Carvalho
14. Computational Modeling of Mitochondrial Function from a Systems Biology Perspective Sonia Cortassa, Steven J. Sollott, and Miguel A. Aon
15. Monitoring the Mitochondrial Dynamics in Mammalian Cells Luca Simula and Silvia Campello
16. Plate-Based Measurement of Superoxide and Hydrogen Peroxide Production by Isolated Mitochondria Hoi-Shan Wong, Pierre-Axel Monternier, Adam L. Orr, and Martin D. Brand
17. Plate-Based Measurement of Respiration by Isolated Mitochondria Shona A. Mookerjee, Casey L. Quinlan, Helen Wong, Pratiksha Dighe, and Martin D. Brand
18. Detection of Iron Depletion- and Hypoxia-Induced Mitophagy in Mammalian Cells Shun-ichi Yamashita and Tomotake Kanki
19. The Importance of Calcium Ions for Determining Mitochondrial Glycerol-3-Phosphate Dehydrogenase Activity When Measuring Uncoupling Protein 1 (UCP1) Function in Mitochondria Isolated from Brown Adipose Tissue Kieran J. Clarke and Richard K. Porter
20. Isolation and Analysis of Mitochondrial Small RNAs from Rat Liver Tissue and HepG2 Cells Julian Geiger and Louise T. Dalgaard
21. Imaging of Mitochondrial pH Using SNARF-1 Venkat K. Ramshesh and John J. Lemasters
22. Relation between Mitochondrial Membrane Potential and ROS Formation Jan Suski, Magdalena Lebiedzinska, Massimo Bonora, Paolo Pinton, Jerzy Duszynski, and Mariusz R. Wieckowski
23. Assessing Spatio-Temporal and Functional Organization of Mitochondrial Networks Felix T. Kurz, Miguel A. Aon, Brian O´Rourke and Antonis A. Armoundas
24. Measurement of Mitochondrial ROS Formation Soni Deshwal, Salvatore Antonucci, Nina Kaludercic, and Fabio Di Lisa

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