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This volume provides an introduction for the scientist who is new to the field of molecular imaging, as well as detailed methods for experts in other areas of molecular imaging. Chapters detail the advantages and limitations of combining fluorescent, bioluminescent and radioisotopic, creating dual modality imaging reporter gene construct, bioluminescent imaging, and bioluminescent or fluorescent imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Reporter Gene Imaging : Methods and Protocols aims to ensure successful results in the further study of this vital field.

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1. Dual Modality Imaging of Promoter Activity as a Surrogate for Gene Expression and Function

Ajit Dhadve, Bhushan Thakur, and Pritha Ray

2. Construction of Dual Modality Optical Reporter Gene Constructs for Bioluminescent and Fluorescent Imaging

Ajit Dhadve, Bhushan Thakur, and Pritha Ray

3. Optical Bioluminescence Protocol for Imaging Mice

David Stout and John David

4. Dual Reporter Bioluminescence Imaging with NanoLuc and Firefly Luciferase

Anne E. Gibbons, Kathryn E. Luker, and Gary D. Luker

5. Reporter Based BRET Sensors for Measuring Biological Functions In Vivo

Maitreyi Rathod, Arijit Mal, and Abhijit De

6. Fluorescence Imaging of Mycobacterial Infection in Live Mice Using Fluorescent Protein-Expressing Strains

Ying Kong and Jeffrey Cirillo

7. In vivo Bacterial Imaging using Bioluminescence

Mariette Barbier, Justin Bevere, and F. Heath Damron

8. Production of GFP and Luciferase Expressing Reporter Macrophages for In Vivo Bioluminescence Imaging

Jukka Pajarinen, Tzu-hua Lin, and Stuart B. Goodman

9. Hypoxia-induced Reporter Genes with Different Half-lives

Balaji Krishnamachary, Pierre Danhier, Samata Kakkad, Santosh K. Bharti, and Zaver M. Bhujwalla

10. Visualization of Immune System Reconstitution by Bioluminescent Imaging

Purnima Dubey

11. Thymidine Kinase PET Reporter Gene Imaging of Cancer Cells In Vivo

Melissa M. McCracken

12. Ex vivo Radiolabeling and In Vivo PET Imaging of T Cells Expressing Nuclear Reporter Genes

Maxim A. Moroz, Pat Zanzonico, Jason T. Lee, and Vladimir Ponomarev

13. Imaging T Cell Dynamics and Function by PET using Human Nuclear Reporter Genes

Jason T. Lee, Maxim A. Moroz, and Vladimir Ponomarev

14. Positron Emission Tomography Imaging of Tumor Apoptosis with a Caspase-sensitive Nano-Aggregation Tracer [ 18 F]C-SNAT

Zimin Chen and Jianghong Rao

15. Practical Guidelines for Cerenkov Luminescence Imaging with Clinically Relevant Isotopes

Nikunj B. Bhatt, Darpan N. Pandya, William A. Dezarn, Frank C. Marini, Dawen Zhao, William H. Gmeiner, Pierre L. Triozzi, and Thaddeus J. Wadas

16. Synthesis, Purification, Characterization, and Imaging of Cy3-Functionalized Fluorescent Silver Nanoparticles in 2D and 3D Tumor Models

Jessica Swanner and Ravi Singh

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