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This volume provides a wide range of methods and protocols detailing various protein structures as platforms for building architectures with targeted application. Chapters guide the readers through exploiting a number of protein scaffolds including virus nanoparticles, elastin and collagen peptides and proteins, and other protein templates for either building materials or presentation of ligands. Site-specific bioconjugation methods, some unique protein architectures, and techniques that exploit peptide amphiphile micelles and assembly of chaperones are also featured. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Protein Scaffolds: Design, Synthesis, and Applications aims to ensure successful results in the study of this vital field.

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1. Nanoreactors via Encapsulation of Catalytic Gold Nanoparticles within Cowpea Chlorotic Mottle Virus

Aijie Liu, Mark V. de Ruiter, Stan J. Maassen, and Jeroen J.L.M. Cornelissen

2. Encapsulation of Active Enzymes within Bacteriophage P22 virus-like Particles

Dustin P. Patterson

3. Encapsulation of Exogenous Proteins in Vault Nanoparticles

Meng Wang, Danny Abad, Valerie A. Kickhoefer, Leonard H. Rome, and Shaily Mahendra

4. Enzyme Encapsulation in an Engineered Lumazine Synthase Protein Cage

Yusuke Azuma and Donald Hilvert

5. Metal Ion-induced Self-Assembly and Packaging of CCMV Nanocapsules

Lise Schoonen, Mark B. van Eldijk, and Jan C. M. van Hest

6. Modification of CCMV Nanocages for Enzyme Encapsulation

Lise Schoonen and Jan C. M. van Hest

7. Synthesis of Cross-linked 2-aminoethyl Methacrylate in P22 Viral Capsid via Atom-transfer Radical Polymerization

Shefah Qazi

8. Synthesis of Metal-organic Frameworks on Tobacco Mosaic Virus Templates

Shaobo Li and Jeremiah J. Gassensmith

9. TMV Disk Scaffolds for Making sub-30 nm Silver Nanorings

Serene Bayram, Omar Zahr, Julia Del Re, and Amy Szuchmacher Blum

10. TMV Disk Scaffolds for Making sub-30 nm Silver Nanorings

Serene Bayram, Omar Zahr, Julia Del Re, and Amy Szuchmacher Blum

11. Synthesis of Triangular Silver and Gold Nanoprisms Using Consensus Sequence Tetratricopeptide Repeat Proteins

Xi Geng and Tijana Z. Grove

12. Type III Secretion Filaments as Templates for Metallic Nanostructure Synthesis

Anum Azam Glasgow

13. Incorporation of Non-canonical Amino Acids into Proteins by Global Reassignment of Sense Codons

Katharine Y. Fang, Seth A. Lieblich, and David A. Tirrell

14. Modification of Protein Scaffolds via Copper-catalyzed Azide-Alkyne Cycloaddition

Stanislav Presolski

15. Antibody Modification of p-aminophenylalanine-containing Proteins

Adel M. ElSohly, Chawita Netirojjanakul, and Matthew B. Francis

16. Cross-linked Collagen Gels using Gold Nanoparticles

Katarzyna Slowinska

17. Genipin Cross-linking Of Elastin and Elastin-based Proteins

Lisa D. Muiznieks

18. Characterization of Self-Assembled Protein Scaffolds from Collagen-mimetic Peptides

Devyani Shukla, Jayeeta Lahiri, and Avanish Singh Parmar
19. Design and Characterization of Fibers and Bionanocomposites Using the Coiled-coil Domain of Cartilage Oligomeric Matrix Protein
Priya Katyal and Jin Kim Montclare

20. Preparing Genes for Repetitive Elastin-like Polypeptides Using Gibson Assembly

James K. Deyling, Edward M. Turk, and Nolan B. Holland

21. Peptide Amphiphile Micelles for Vaccine Delivery

John C. Barrett and Matthew V. Tirrell

22. Controlled Assembly of the Filamentous Chaperone Gamma-prefoldin into Defined Nanostructures

Douglas S. Clark and Dominic J. Glover

23. Advances in the Application of Designed Ankyrin Repeat Proteins (Darpins) As Research Tools and Protein Therapeutics

Ykelien L. Boersma

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