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This fully updated volume utilizes the expertise of scientists currently engaged in immunotoxicity testing to provide the reader with lab-ready procedures and the background information needed to identify effective testing approaches. Dedicated to identifying and describing exogenous agents that can modify immune function, uncovering modes and mechanisms of action for such agents, and translating data from the laboratory and from the clinic to better predict health risks as well as benefits to those who are exposed to immunomodulatory agents, immunotoxicity testing continues to be a vital field of study, and this collection highlights both the "tried and true" methods as well as alternative protocols that have been more recently developed. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Immunotoxicity Testing: Methods and Protocols, Second Edition serves as a valuable contribution to the continued evolution and the application of immunotoxicity testing.

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Part I: Overview and Health Risk Considerations

1. Immunotoxicology: A Brief History

Kenneth L. Hastings

2. Clinical Immunotoxicology

L. Peyton Myers

3. Investigative Immunotoxicology

Stacey E. Anderson and Hillary L. Shane

4. Developmental Immunotoxicity (DIT) Testing: Current Recommendations and the Future of DIT Testing

Annalise N. vonderEmbse and Jamie C. DeWitt

5. Markers of Inflammation

Dori R. Germolec, Kelly A. Shipkowski, Rachel P. Frawley, and Ellen Evans

Part II: Legacy Protocols: In Vivo

6. The Sheep Erythrocyte T-Dependent Antibody Response (TDAR)

Gregory S. Ladics

7. Methylated-Bovine Serum Albumin (mBSA)-Induced Delayed-Type Hypersensitivity in Mice

Jessica Margaret Sido

8. Use of the LLNA:BrdU-ELISA for Skin Sensitization Hazard Assessment

David M. Lehmann

9. Host Resistance Assays

Stefanie C.M. Burleson, Wendy Jo Freebern, Florence G. Burleson, Gary R. Burleson, Victor J. Johnson, and Robert W. Luebke

10. Enhanced Histopathology Evaluation of Lymphoid Organs

Susan A. Elmore

11. Tumor Challenges in Immunotoxicity Testing

Sheung Ng and Judith T. Zelikoff

Part III: Legacy Protocols: Ex Vivo and In Vitro

12. Flow Cytometry for the Immunotoxicologist

Scott T. Espenchied, Robert M. Tighe, and Kymberly M. Gowdy

13. Evaluation of Cell-Mediated Immune Function Using the Cytotoxic T Lymphocyte Assay

Gary R. Burleson, Florence G. Burleson, and Rodney R. Dietert

14. Evaluation of Cell Proliferation and Apoptosis in Immunotoxicity Testing

Mitzi Nagarkatti, Sadiye Amcaoglu Rieder, and Prakash S. Nagarkatti

15. Natural Killer (NK) Cell Assays in Immunotoxicity Testing

Qing Li

16. Dendritic Cell Assays

Joanna M. Kreitinger and David M. Shepherd

17. Evaluating Macrophages in Immunotoxicity Testing

Jennifer Franko, Jamie L. McCall, and John B. Barnett

18. Evaluating Cytokines in Immunotoxicity Testing

Emanuela Corsini and Robert V. House

Part IV: Alternative Protocols

19. Functional Assays of Hematopoietic Stem Cells in Toxicology Research
Michael D. Laiosa

20. CD4 + T Cell Differentiation and Activation

Jim Reed and Scott A. Wetzel

21. Isolation and Identification of Innate Lymphoid Cells (ILCs) for Immunotoxicity Testing

Elia D. Tait Wojno and Celine A. Beamer

22. Evaluating Antigen-Specific IgE Using the Rat Basophil Leukemia Cell (RBL) Assay

Marsha D.W. Ward and Lisa B. Copeland

Part V: Emerging Issues

23. Challenges for Integrating Immunotoxicology into the 21 st Century Toxicology Testing Paradigm

Peer W.F. Karmaus and Agnes L. Karmaus

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