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This volume provides a comprehensive overview of different ways to grow algae and the techniques used to start algal cultivation, monitor algal growth, environmental impact of growing algae, and various methods for characterizing the biomass. The chapters in this book discuss a range of topics such as step-by-step protocols on how to isolate and grow algae; descriptions on how to use fluorescence to estimate algal biomass and lipid content; and instructions on using advanced techniques to determine the carbohydrates and lipids in algal biomass. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Biofuels from Algae: Methods and Protocols is a valuable resource for novice and experienced researchers who want to learn more about this impactful and developing field.

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Table of Contents

1: Large Scale Cultivation of Microalgae: Open and Closed Systems
Jacqueline Jerney and Kristian Spilling

2: Design and Analysis of Offshore Macroalgae Biorefineries
Alexander Golberg, Alexander Liberzon, Edward Vitkin, and Zohar Yakhini

3: Basic Methods for Isolating and Culturing Microalgae
Kristian Spilling

4: Measurement of Fluorescence for Monitoring Algal Growth and Health
Kristian Spilling and Jukka Seppälä

5: Microalgae Lipid Staining with Fluorescent BODIPY Dye
Judita Koreivien

6: Monitoring Microalgal Neutral Lipid Accumulation with Nile Red
Katariina Natunen

7: Determining Inorganic and Organic Carbon
Jaana Koistinen, Mervi Sjöblom, and Kristian Spilling

8: Determining Inorganic and Organic Nitrogen
Jaana Koistinen, Mervi Sjöblom, and Kristian Spilling

9: Total Nitrogen Determination by a Spectrophotometric Method
Jaana Koistinen, Mervi Sjöblom, and Kristian Spilling

10: Determining Inorganic and Organic Phosphorus
Jaana Koistinen, Mervi Sjöblom, and Kristian Spilling

11: Determining Dissolved and Biogenic Silica
Jaana Koistinen, Mervi Sjöblom, and Kristian Spilling

12: Life Cycle Assessment of Seaweed Cultivation Systems
Michele Seghetta and Pietro Goglio

13: Life Cycle Analysis in Algae Fuels
Homa Hosseinzadeh-Bandbafha, Meisam Tabatabaei, Mortaza Aghbashlo, Alawi Sulaiman, and Abbas Ghassemi

14: Metabolic Engineering for Microalgae for Biofuels Production
Mohammad Pooya Naghshbandi, Meisam Tabatabaei, Mortaza aghbashlo, Muhammad Nauman aftab, and Irfana Iqbal

15: Analytical Grade Purification of Phycocyanin from Cyanobacteria
Mahammed Ilyas Khazi, Zeliha Demirel, Fakhra Liaqat, and Meltem Conk Dalay

16: Complete Acid Based Hydrolysis Assay for Carbohydrate Quantification in Seaweed: A Species Specific Optimized Approach
Emily T. Kostas, Stuart J. Wilkinson, Daniel A. White, and David J. Cook

17: Total Carbohydrate Content Determination of Microalgal Biomass by Acid Hydrolysis followed by Spectrophotometry or Liquid Chromatography
S. Van Wychen and L. ML. Laurens

18: Total Fatty Acid Content Determination of Whole Microalgal Biomass using In Situ Transesterification
S. Van Wychen and L. ML. Laurens

19: Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Analysis of Molecular Lipids in Algae Samples
Heli Nygren, Tuulikki Seppänen-Laakso, and Heiko Rischer

20: UPLC-ELSD Analysis of Algal Lipid Classes and Derivatization of Bound and Free Fatty Acids and Sterols for GC-MS Methods
Tuulikki Seppänen-Laakso, Heli Nygren, and Heiko Rischer

21: Total Protein Content Determination of Microalgal Biomass by Elemental Nitrogen Analysis and a Dedicated Nitrogen-To-Protein Conversion Factor
L. ML. Laurens. J. L. Olstad, and D. W. Templeton

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