---

Haemorrhagic septicaemia (HS) is considered as a major and one of the most important infectious diseases of cattle and buffalo. In some countries, particularly eastern Asian countries, animals play an important integrated role in rural communities as a main source of milk, fuel, meat and fertiliser. HS due to P.multocida serotype B, causes loss of millions of dollars to the farming industry worldwide annually. In order to control and minimise the losses, wide range of preventive measures, predominantly in vaccine development against HS has been carried out (Dabo et al., 2008). There have been several types of vaccines developed with very limited protection. The clone ABA392 used in this study is derived from a P. multocida serotype B 202, carrying a recombinant plasmid. This clone was constructed by shotgun cloning method using E.coli as host. This recombinant plasmid harbours a sequence that code for a virulence factor to P. multocida. The aim of this study was to determine the immunogenicity of the clone, sub-cloning into an expression system and its potency as possible vaccine candidate.

---

BSc. Medical Lab Technology, MSc. Medical Microbiology, (Manipal Academy of Higher Education, Manipal, India). PhD. Molecular Bacteriology (University of Malaya, Kuala Lumpur, Malaysia). Research field and expertise: Molecular bacteriology, immunogenicity & virulence study of various cloning products in laboratory animals.

---