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Proceedings of the NATO Advanced Study Institute "4th International Course on Flow Cytometry" held at Villejuif, France, from April 6-10, 1992

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Described here are the practical applications of flow cytometry in specific biological systems, ranging from cell biology to chromosome analysis and sorting. Three major areas of interest in cell and molecular biology are addressed: - Cell Activation and Biological Response. - Membrane-Ligand Interactions and Cell Identity. - Nuclear Components: Form and Function. Data management, expert systems and cell sorting techniques concerning all aspects of flow cytometry are also presented.

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I Cell Activation and Biological Response Modifiers.- Cytosolic Events Related to Cell Activation: Potential Interaction Between Signal and Energy Transduction Pathways in Smooth Muscle Cells.- Studies of Changes in Cytoplasmic pH and Membrane Potential Using Flow Cytometry.- Measurement of Enzyme Kinetics by Flow Cytometry.- Endocytosis Analysis by Flow Cytometry: Study of the Endocytosis of Fluoresceinylated Neoglycoproteins via Membrane Lectins of Macrophages.- Analysis of Intracellular Antigens by Flow Cytometry: Methods for Cell Permeabilization to Antibodies.- Expression and Role of Surface Markers of Immunocompetent Cells in Non-lympho‹d Systems.- Change in Lipid Composition and in Membrane Fluidity of Human Peripheral Blood Lymphocytes undergoing Blastic Transformation.- II: Membrane - Ligand Interactions and Cell Identity.- Cellular Proliferation History by Fluorescent Analysis.- Identification and Isolation of Bone Marrow Stem Cells.- T.Lymphocytes Recognition Molecules in Homing: A Flow Cytometry Study of Lectin-glycoconjugates Interactions.- Three- and Four-color Immunofluorescence Analysis by Flow Cytometry.- Energy Transfer.- Fluid Mechanical Properties of Flow Cytometers and Assessment Cell-cell Adhesion Forces.- Use of Biotin-labeled Growth Factors for Receptor Studies.- Cytometric Tout Terrain or Bush DNA Cytometry.- III: Nuclear Components: Form and Function.- Bromodeoxuridine Procedures for Analysis of DNA Synthesis.- Flow Cytogenetics: Fundamentals and New Developments.- In situ Hybridization.- The Molecular Basis of Mammalian Cell Growth Control.- Flow Cytometric Analysis of Nuclear Associated Antigens.- Chromatin Changes Accompanying Apoptosis Detected by Flow Cytometry.- Gene Mapping and PCR Applications with Flow-sorted Chromosomes.- Cytometry, Antitumour Drugs and DNA Topoisomerases.- Detection of Apoptosis Using Fluorescent In Situ Nick Translation.- IV: Data Management Expert Systems and Cell Sorting Techniques.- Flow Cytometry Data Analysis. Basic Concepts and Analysis of Mono-dimensional Immunofluorescence Histograms.- Analysis of Multiparameter Flow Cytometric Data.- Expert Assistance for Flow Immunophenotyping.- DNA Content and Cell Cycle Analysis by Flow Cytometry in Clinical Samples: Applications in Cancer.- Immunomagnetic Particles for Cell Isolation.- Optical Trapping: Instrumentation and Biological Applications.

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