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Ein umfassendes Kompendium für alle, die mit kleinen oder großen RNAs arbeiten. Bietet Hunderte von Protokollen, umfassende Erläuterungen und Tipps zur Fehlerbehebung, die in keinem anderen Nachschlagewerk zu finden sind.

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The second edition of a highly acclaimed handbook and ready reference.
Unmatched in its breadth and quality, around 100 specialists from all over the world share their up-to-date expertise and experiences, including hundreds of protocols, complete with explanations, and hitherto unpublished troubleshooting hints.
They cover all modern techniques for the handling, analysis and modification of RNAs and their complexes with proteins. Throughout, they bear the practising bench scientist in mind, providing quick and reliable access to a plethora of solutions for practical questions of RNA research, ranging from simple to highly complex.
This broad scope allows the treatment of specialized methods side by side with basic biochemical techniques, making the book a real treasure trove for every researcher experimenting with RNA.

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RNA SYNTHESIS
Enyzmatic synthesis, ligation and modification
Chemical synthesis
NEW: RNA detection and quantitation
STRUCTURE DETERMINATION
Sequence determination
NEW: Probing in vitro and in vivo
Footprinting techniques
Nucleotide analog interference mapping
Identification of interacting proteins and nucleic acids
Spectroscopic studies of RNA structure
Atomic structure determination
NEW: Functional assays
NEW: Mass spectrometry of RNA
NEW: Folding and unfolding of single RNA molecules
RNA GENOMICS & BIOINFORMATICS
Phylogenetic analysis
Structure prediction
Modeling of RNA structures
Molecular dynamics simulation
NEW: Searching and predicting small RNAs
NEW: RNA mining and RNomics in eukaryotes
NEW: Exon arrays
ANALYSIS OF RNA-PROTEIN INTERACTION
Isolation and purification of RNA-binding proteins
Sedimentation analysis of RNP complexes
Protein-RNA crosslinking
Immunoaffinity purification
NEW: TAP-tag affinity purification
Three-hybrid analysis to identify RNA-binding proteins
SELEX
In vitro selection of ribozymes and aptamers
In vivo SELEX
In vitro selection against small targets
NEW: Genomic SELEX
RNA INTERFERENCE
NEW: Chemical modification of siRNA
NEW: siRNA design and delivery
NEW: RNAi in mammalian cells
(partial contents)

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