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This monograph reviews all relevant technologies based on mass spectrometry that are used to study or screen biological interactions in general.
Arranged in three parts, the text begins by reviewing techniques nowadays almost considered classical, such as affinity chromatography and ultrafiltration, as well as the latest techniques. The second part focusses on all MS-based methods for the study of interactions of proteins with all classes of biomolecules. Besides pull down-based approaches, this section also emphasizes the use of ion mobility MS, capture-compound approaches, chemical proteomics and interactomics. The third and final part discusses other important technologies frequently employed in interaction studies, such as biosensors and microarrays.
For pharmaceutical, analytical, protein, environmental and biochemists, as well as those working in pharmaceutical and analytical laboratories.

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Preface
INTRODUCTION TO MASS SPECTROMETRY, A TUTORIAL
Introduction
Figures of Merit
Analyte Ionization
Mass Analyzer Building Blocks
Tandem Mass Spectrometry
Data Interpretation and Analytical Strategies
Conclusion and Perspectives
PART I: Direct MS Based Affinity Techniques
STUDYING PROTEIN-PROTEIN INTERACTIONS BY COMBINING NATIVE MASS SPECTROMETRY AND CHEMICAL CROSS-LINKING
Introduction
Protein Analysis by Mass Spectrometry
Native MS
Chemical Cross-Linking MS
Value of Combining Native MS with Chemical Cross-Linking MS
Regulating the Giant
Capturing Transient Interactions
An Integrative Approach for Obtaining Low-Resolution Structures of Native Protein Complexes
Future Directions
NATIVE MASS SPECTROMETRY APPROACHES USING ION MOBILITY-MASS SPECTROMETRY
Introduction
Sample Preparation
Electrospray Ionization
Mass Analyzers and Tandem MS Approaches
Ion Mobility
Data Processing
Challenges and Future Perspectives
PART II: LC-MS Based with Indirect Assays
METHODOLOGIES FOR EFFECT-DIRECTED ANALYSIS: ENVIRONMENTAL APPLICATIONS, FOOD ANALYSIS, AND DRUG DISCOVERY
Introduction
Principle of Traditional Effect-Directed Analysis
Sample Preparation
Fractionation for Bioassay Testing
Miscellaneous Approaches
Identification of Confirmation Process
Conclusion and Perspectives
MS BINDING ASSAYS
Introduction
MS Binding Assays - Strategy
Application of MS Binding Assays
Summary and Perspectives
METABOLIC PROFILING APPROACHES FOR THE IDENTIFICATION OF BIOACTIVE METABOLITES IN PLANTS
Introduction to Plant Metabolic Profiling
Sample Collection and Processing
Hyphenated Techniques
Mass Spectrometry
Mass Spectrometric Imaging
Data Analysis
Future Perspectives
ANTIVENOMICS: A PROTEOMICS TOOL FOR STUDYING THE IMMUNOREACTIVITY OF ANTIVENOMS
Introduction
Challenge of Fighting Human Envenoming by Snakebites
Toolbox for Studying the Immunological Profile of Antivenoms
First-Generation Antivenomics
Snake Venomics
Second-Generation Antivenomics
Concluding Remarks
PART III: Direct Pre- and On-Column Coupled Techniques
FRONTAL AND ZONAL AFFINITY CHROMATOGRAPHY COUPLED TO MASS SPECTROMETRY
Introduction
Frontal Affinity Chromatography
Staircase Method
Simultaneous Frontal Analysis of a Complex Mixture
Multiprotein Stationary Phase
Zonal Chromatography
Nonlinear Chromatography
ONLINE AFFINITY ASSESSMENT AND IMMUNOAFFINITY SAMPLE PRETREATMENT IN CAPILLARY ELECTROPHORESIS-MASS SPECTROMETRY
Introduction
Capillary Electrophoresis
Affinity Capillary Electrophoresis
Immunoaffinity Capillary Electrophoresis
Capillary Electrophoresis-Mass Spectrometry
Application of ACE-MS
Applications of IA-CE-MS
Conclusions
LABEL-FREE BIOSENSOR AFFINITY ANALYSIS COUPLED TO MASS SPECTROMETRY
Introduction to MS-Coupled Biosensor Platforms
Strategies for Coupling Label-Free Analysis with Mass Spectrometry
New Sensor and MS Platforms, Opportunities for Integration
PART IV: Direct Post Column Coupled Affinity Techniques
HIGH-RESOLUTION SCREENING: POST-COLUMN CONTINUOUS-FLOW BIOASSAYS
Introduction
The High-Resolution Screening Platform
Data Analysis
Conclusions and Perspectives
CONCLUSIONS
Index

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