This book provides an information about the standardization of in-vitro regeneration protocol in brinjal (Solanum melongena L.) through direct and indirect organogenesis. This book also provides a subsequent information about standardization of Agrobacterium tumefacience mediated genetic transformation system and the factors influencing genetic transformation process to develop moisture stress tolerance in brinjal using DREB1A:rd29A gene.Deepti B. Sagare, M.Sc. (Ag) Agriculturel Biotechnology. Ph.D. Scholar at Institute of Biotechnology, PJTSAU, Hyderabad - 500030 (India)