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Recent developments in microarray technology have changed the landscape of biology and biomedical research, and they have revolutionized RNA and DNA research. In Biological Microarrays: Methods and Protocols, expert researchers explore exciting new developments in the field, providing a comprehensive approach to biological microarrays that conveys not only the state-of-the-art fundamentals, but also includes applications of the most innovative methods. Chapters address both the application of biological microarrays, including DNA/RNA, apatmer, proteins, tissues, oligonucleotides, carbohydrates, biomaterials, cells, bacteria, and virus microarrays, and also explore the different techniques used for generating microarray platforms. Composed in the highly successful Methods in Molecular Biology(TM) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Wide-ranging and revolutionary, Biological Microarrays: Methods and Protocols serves as a primary source for academics, practitioners, and professionals in related fields, including biologists, biotechnologists, biochemists, analytical chemists, and biomedical, physical, and microsystems engineers, to name a few, appealing to all of those interested in the present and future state of biological microarray research.

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Biological Microarrays: Methods and Protocols
Editors: Ali Khademhosseini, Suh Kahp-Yang, Mohammed Zourob

Table of Contents

Preface

Contributors

Part 1: Application of Biological Microarray

1. RNA and DNA Microarrays
Tearina T. Chu and Stuart C. Sealfon

2. Aptamer Arrays
Eva Baldrich

3. Oligonucleotide microarrays for identification of microbial pathogens and detection of their virulence-associated or drug-resistance determinants
Dmitriy V. Volokhov, Hyesuk Kong, Keith Herold, Vladimir E. Chizhikov, and Avraham Rasooly

4. Protein MicroArrays printed from DNA MicroArrays
Oda Stoevesandt, Mingyue He and Michael J Taussig

5. Lithographically-defined two- and three-dimensional tissue microarrays
Esther W. Gomez and Celeste M. Nelson

6. Ratiometric lectin microarray analysis of the mammalian cell surface glycome
Ku-Lung Hsu, Kanoelani Pilobello, Lakshmipriya Krishnamoorthy, and Lara K. Mahal

7. Cell microarrays based on hydrogel microstructures for the application to cell-based biosensor
Won-Gun Koh
8. Fabrication of Bacteria and Virus Microarrays Based on Polymeric Capillary Force Lithography
Pil J. Yoo

9. 3D Polymer Scaffold Arrays
Carl G. Simon, Jr., Yanyin Yang, Shauna M. Dorsey, Murugan Ramalingam, Kaushik Chatterjee

Part 2: Methods for Microarray Generation
10. PDMS microfluidic capillary systems for patterning proteins on surfaces and performing miniaturized immunoassays
Mateu Pla-Roca and David Juncker

11. Merging Photolithography and Robotic Protein Printing to Create Cellular Microarrays
Ji Youn Lee and Alexander Revzin

12. Generation of Protein and Cell Microarrays on Functionalized Surfaces
Yoo Seong Choi and Chang-Soo Lee

13. Microprinting of Liver Micro-organ for Drug Metabolism Study
Robert C. Chang, Kamal Emami, Antony Jeevarajan, Honglu Wu, Wei Sun

14. Microcontact printing
Yunyan Xie and Xingyu Jiang

15. Micromolding for the Fabrication of Biological Microarrays
Ashley L. Galloway, Andrew Murphy, Jason P. Rolland, Kevin P. Herlihy, Robby A. Petros, Mary E. Napier, and Joseph M. DeSimone

16. Progress Report on Microstructured Surfaces Based on Chemical Vapor Deposition
Yaseen Elkasabi and Joerg Lahann

17. Methods for Forming Human Microvascular Tubes in Vitro and Measuring Their Macromolecular Permeability
Gavrielle M. Price and Joe Tien

18. Microarray Bioinformatics
Robert P. Loewe and Peter J. Nelson

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