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This book offers in a single volume a unique collection of the state-of the-art experimental procedures utilized for the induction, detection, and modeling of this complex cellular program of oncogene-induced senescence. The book encompasses protocols for studying this multi-step program in human specimens and a variety of experimental models including cultured mammalian cells, laboratory mice, and Drosophila melanogaster , as well as offering a description of high throughput approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Oncogene-Induced Senescence: Methods and Protocols represents a valuable asset for a wide audience of medical oncologists and researchers in the fields of oncology, molecular and cellular biology, biochemistry, and animal development.

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1. The Immortal Senescence

Anna Bianchi-Smiraglia, Brittany C. Lipchick, and Mikhail A. Nikiforov

2. Senescence Phenotypes Induced by Ras in Primary Cells

Lena Lau and Gregory David

3. Cellular Model of p21-Induced Senescence

Michael Shtutman, Bey-Dih Chang, Gary P. Schools, and Eugenia V. Broude

4. Detecting Markers of Therapy-Induced Senescence in Cancer Cells
Dorothy N.Y. Fan and Clemens A. Schmitt

5. Genome-Wide miRNA Screening for Genes Bypassing Oncogene-Induced Senescence

Maria V. Guijarro and Amancio Carnero

6. Detection of Dysfunctional Telomeres in Oncogene-Induced Senescence

Priyanka L. Patel and Utz Herbig

7. RT-qPCR Detection of Senescence-Associated Circular RNAs
Amaresh C. Panda, Kotb Abdelmohsen, and Myriam Gorospe

8. Autophagy Detection During Oncogene-Induced Senescence Using Fluorescence Microscopy

Masako Narita and Masashi Narita

9. Detecting the Senescence-Associated Secretory Phenotype (SASP) by High Content Microscopy Analysis

Priya Hari and Juan Carlos Acosta

10. Sudan Black B, the Specific Histochemical Stain for Lipofuscin: A Novel Method to Detect Senescent Cells
Konstantinos Evangelou and Vassilis G. Gorgoulis

11. Using [U- 13 C 6 ]-Glucose Tracer to Study Metabolic Changes in Oncogene-Induced Senescence Fibroblasts

Katerina I. Leonova and David A. Scott

12. Detection of the Ubiquitinome in Cells Undergoing Oncogene-Induced Senescence

Hengrui Zhu, Linh Le, Hsin-Yao Tang, David W. Speicher, and Rugang Zhang

13. Detection of Reactive Oxygen Species in Cells Undergoing Oncogene-Induced Senescence

Rabii Ameziane-El-Hassani and Corinne Dupuy

14. Detection of Senescent Cells by Extracellular Markers Using a Flow Cytometry-Based Approach

Mohammad Althubiti and Salvador Macip

15. Metabolic Changes Investigated by Proton NMR Spectroscopy in Cells Undergoing Oncogene-Induced Senescence

Claudia Gey and Karsten Seeger

16. Detection of Nucleotide Disbalance in Cells Undergoing Oncogene-Induced Senescence

Mikhail A. Nikiforov and Donna S. Shewach

17. Senescence-Like Phenotypes in Human Nevi

Andrew Joselow, Darren Lynn, Tamara Terzian, and Neil F. Box

18. Detection of Oncogene-Induced Senescence In Vivo

Kwan-Hyuck Baek and Sandra Ryeom
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19. Detection of Senescence Markers During Mammalian Embryonic Development

Mekayla Storer and William M. Keyes

20. Induction and Detection of Oncogene-Induced Cellular Senescence in Drosophila

Mai Nakamura and Tatsushi Igaki

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