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This volume presents methods and protocols on modern analysis of steroid receptor and prostate cancer function. Chapters detail androgen, androgen receptor action, anti-androgen enzalutamide, in vitro and in vivo models, imaging, gene fusions, and help facilitate studies with novel drugs including anti-androgens and chemotherapeutics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Prostate Cancer: Methods and Protocols aims to provide easily reproducible techniques.

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1. Generation of Prostate Cancer Patient-derived Xenografts to Investigate Mechanisms of Novel Treatments and Treatment Resistance

Hung-Ming Lam, Holly M Nguyen, and Eva Corey

2. Methods to Study Angiogenesis in a Mouse Model of Prostate Cancer

Ana-Rita Pedrosa, Alexandre Trindade, and António Duarte

3. Methodologies Applied to Establish Cell Cultures in Prostate Cancer

Anne T Collins

4. Protocols for Migration and Invasion Studies in Prostate Cancer

Arjanneke F. van de Merbel, Geertje van der Horst, Jeroen T. Buijs, and Gabri van der Pluijm
5. Transplantable Animal Studies and Whole-body Optical Imaging in Prostate Carcinoma
Geertje van der Horst, Maaike van der Mark, Henry Cheung, and Gabri van der Pluijm

6. Protocols for Tissue Microarrays in Prostate Cancer Studies

Tatjana Vlajnic, Serenella Eppenberger-Castori, and Lukas Bubendorf

7. Functional Studies on Steroid Receptors

Simon Schlanger and Hannelore V. Heemers

8. Protocols for Studies on TMPRSS/ERG in Prostate Cancer

Hubert Pakula, Douglas E. Linn, Daniel R. Schmidt, Marit Van Gorsel, Matthew G. Vander Heiden, and Zhe Li

9. Protocols for the Study of Taxanes Chemosensitivity in Prostate Cancer

M. Luz Flores and Carmen Sáez

10. A Method for Prostate and Breast Cancer Cell Spheroid Cultures using Gelatin Methacryloyl-based Hydrogels

Christoph Meinert, Christina Theodoropoulos, Travis J Klein, Dietmar W Hutmacher, and Daniela Loessner

11. Protocols for Studies on Genetically Engineered Mouse Models in Prostate Cancer

Chris W.D. Armstrong, Oksana Lyubomska, Melissa J. LaBonte, and David J.J. Waugh

12. Protocols for Studies on Stromal Cells in Prostate Cancer

Damien A. Leach and Grant Buchanan

13. Techniques for Evaluation of AR Transcriptional Output and Recruitment to DNA

Manqi Zhang, William C Krause, and Irina U Agoulnik
14. NMR-based Prostate Cancer Metabolomics
Leslie R. Euceda, Maria K. Andersen, May-Britt Tessem, Siver A. Moestue, Maria T. Grinde, and Tone F. Bathen

15. Studies on Steroid Receptor Coactivators in Prostate Cancer

Zoran Culig and Frédéric R. Santer

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