---

A review of innovative tools for creative nucleic acid chemists that open the door to novel probes and therapeutic agents

Nucleic acids continue to gain importance as novel diagnostic and therapeutic agents. With contributions from noted scientists and scholars, Enzymatic and Chemical Synthesis of Nucleic Acid Derivatives is a practical reference that includes a wide range of approaches for the synthesis of designer nucleic acids and their derivatives.

The book covers enzymatic (including chemo-enzymatic) methods, with a focus on the synthesis and incorporation of modified nucleosides. The authors also offer a review of innovative approaches for the non-enzymatic chemical synthesis of nucleic acids and their analogs and derivatives, highlighting especially challenging species. The book offers a concise review of the methods that prepare novel and heavily modified polynucleotides in sufficient amount and purity for most clinical and research applications. This important book:

-Presents a timely and topical guide to the synthesis of designer nucleic acids and their derivatives
-Addresses the growing market for nucleotide-derived pharmaceuticals used as anti-infectives and chemotherapeutic agents, as well as fungicides and other agrochemicals.
-Covers novel methods and the most recent trends in the field
-Contains contributions from an international panel of noted scientistics

Written for biochemists, medicinal chemists, natural products chemists, organic chemists, and biotechnologists, Enzymatic and Chemical Synthesis of Nucleic Acid Derivatives is a practice-oriented guide that reviews innovative methods for the enzymatic as well as non-enzymatic synthesis of nucleic acid species.

---

Enzymatic Synthesis of Nucleoside Analogues by Nucleoside Phosphorylases
Enzymatic Phosphorylation of Nucleosides
Enzymatic Synthesis of Nucleic Acid Derivatives Using Whole Cells
Enzymatic Synthesis of Nucleic Acid Derivatives by Immobilized Cells
Enzymatic Synthesis of Nucleic Acid Derivatives by Immobilized Enzymes
Synthesis of Nucleic Acid Derivatives by Multi-enzymatic Systems
Enzymatic Synthesis Using Polymerases of Modified Nucleic Acids and Genes
Synthetic Approaches to the Fleximer Class of Nucleosides: A Historic Perspective
Synthesis of Oligonucleotides Carrying Nucleic Acid Derivatives of Biomedical and Structural Interest
Synthesis of Carbohydrate-Oligonucleotide Conjugates and their Applications
Advances in Light-directed Synthesis of High-density Microarrays and Extension to RNA and 2´F-ANA Chemistries
SAMHD1-Mediated Negative Regulation of Cellular dNTP Levels: HIV-1, Innate Immunity and Cancers

---

Jesús Fernández Lucas, PhD, is a tenured professor at the European University of Madrid, where he leads the Applied Biotechnology research group. His research is focused on the design and application of novel biocatalysts for the enzymatic synthesis of nucleosides. María José Camarasa Rius, PhD, is Full Professor at the Medicinal Chemistry Institute of the Spanish National Research Council (IQM-CSIC) in Madrid, working on the design of novel enzymatic inhibitors with potential anticancer, antifungal and antiviral activity.

---